Crystal Taq™ PCR Master Mix (2x)


Regular price $109.00

The world’s first Taq polymerase free from background bacterial animal DNA contamination, following a breakthrough in enzyme production.

● Engineered for highly sensitive applications; qPCR and RT-PCR
● High yield and zero contamination, insuring sample longevity
● Cleaner Amplicons for Pyrosequencing
● This enzyme is from a recombinant source

100 Cycle Certification
The enzyme does not show any nonspecific endo- or exo-nuclease activities. The result of 16S assay on our Crystal Taq™ DNA polymerase showed no bacterial DNA contamination after 100 PCR cycles.

 

Applications
● Gene amplification (PCR) and Sequencing
● RT-PCR
● Pyrosequencing and Sanger sequencing
● Diagnostics

Benefits
● The lack of background bacterial and animal DNA will minimize non-specific amplification of target regions. Current levels of contamination in commercial competitor reagents reduce the quality and accuracy of whole genome sequencing. The technology behind Crystal Taq will greatly increase the efficiency of random genome amplification for sequencing.
● Higher sensitivity will increase overall accuracy. Crystal Taq will reduce the overall need for repetitive testing. Required fluorescent dye for real time PCR not included, but available for purchase.
● Crystal Taq will create cleaner amplicons resulting in a higher resolution sequencing result.
● Dramatically reduces false positive rates. Lack of background bacterial and animal DNA will increase the accuracy of diagnostics tests.

Detailed Description
Crystal Taq DNA Polymerase is from Thermus aquaticus. It is a thermostable enzyme with 5’-3’ DNA polymerase activity and 5'-3’ exonuclease activity, while lacking 3’-5’  exonuclease (proofreading) activity. The enzyme is free from bacterial and animal DNA. This first truly pure enzyme will greatly aid new generations of sequencing technologies, while being a superior component to pathogen testing in Clinical Medicine, Food-Borne contamination, Quality Assurance, Forensics, Water Treatment, Veterinary & Agriculture and Bioterrorism.

Packaging: 5 units /μL
Storage Temperature: -25°C to -15°C

Taq Storage Buffer:
20   mM Tris-HCl, pH 8.0 at 25°C
100 mM KCl
0.1  mM EDTA
1     mM DTT
0.5% Tween-20
0.5% IGEPAL CA-630
50% Glycerol

Master Mix Reaction Buffer (1x):
10   mM Tris-HCl, pH 8.3 at 25°C
50   mM KCl
1.5* mM MgCl2

Component Volume Final Concentration
2X Master Mix 10 µl 1X
3 µM Forward Primer 2 µl 0.3 µM
3 µM Reverse Primer 2 µl 0.3 µM
Template DNA Varies < 20 ng/µl
Water Adjust for 20 µl final volume  

 

PCR cycling set-up (25-35 cycles)
Initial Denaturation 95˚C for 2 min
Denaturation 95˚C for 30 sec
Annealing 50˚- 68˚C for 30 sec
Extension 72˚C for 1 min/kb
Final Extension 72˚C for 5 min
Hold 4˚C indefinitely