Samurai Taq™ DNA Polymerase

$126.00

A highly purified, low DNA Taq polymerase, Samurai Taq™ is designed for a wide range of gene amplification applications. Intended for academic and research use, the Taq polymerase is priced affordably for students, teachers, and researchers.

Features
● Low DNA background perfect for gene amplification
● Best Research Value - highly purified DNA Taq polymerase – minimal DNA

Applications
● Gene Amplification (PCR) and Sequencing
● RT-PCR
● Pyrosequencing and Sanger Sequencing
● Diagnostics*

Detailed Description
Samurai Taq™ DNA Polymerase is from Thermus aquaticus. It is a thermostable enzyme with 5’-3’ DNA polymerase activity and 5'-3’ exonuclease activity, while lacking 3’-5’ exonuclease (proofreading) or endo- activity. This enzyme is a recombinant protein produced and purified from an E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1. 

Packaging: 5 units /μL
Storage Temperature: -25°C to -15°C

Taq Storage Buffer:
20   mM Tris-HCl, pH 8.0 at 25°C
100 mM KCl
0.1  mM EDTA
1     mM DTT
0.5% Tween-20
0.5% IGEPAL CA-630
50% Glycerol

PCR Buffer (10x):
200   mM Tris-HCl, pH 8.3 at 25°C
500   mM KCl
15     mM MgCl2

  50 Reaction Volume Final Concentration
Water (ddH2O) adjust for 50 µl final volume  
10X PCR buffer 5 µl 1X
10 mM dNTPs 1 µl 0.2 mM of each
10 µM Forward primer 1.5 µl 0.3 µM
10 µM Reverse primer 1.5 µl 0.3 µM
Template DNA Varies < 0.02 µg/µl
Taq DNA polymerase .5  µl 2.5 U/50 µl

 

PCR cycling set-up (25-35 cycles)
Initial Denaturation 95˚C for 2 min
Denaturation 95˚C for 30 sec
Annealing 50˚- 68˚C for 30 sec
Extension 72˚C for 1 min/kb
Final Extension 72˚C for 5 min
Hold 4˚C indefinitely

 

Notes: DNA templates should preferably be pure. Chemical agents, ionic detergents (such as SDS) and other DNA damaging agents should be avoided. For routine applications, 25 to 35 PCR cycles is usually enough to provide sufficient copies of the target sequence.

*Usage: This product is for research use only in molecular biology applications and is not yet intended for use in diagnostic procedures. Purchase of this product does not include a license to perform any patented application; therefore it is the users responsibility to determine if any license agreement might be needed prior usage of this product. Practicing real-time require additional licensing from Roche or Applied Biosystems.