A highly purified, low DNA Taq polymerase, Samurai Taq™ is designed for a wide range of gene amplification applications. Intended for academic and research use, the Taq polymerase is priced affordably for students, teachers, and researchers.
Features
● Low DNA background perfect for gene amplification
● Best Research Value - highly purified DNA Taq polymerase – minimal DNA
Applications
● Gene Amplification (PCR) and Sequencing
● RT-PCR
● Pyrosequencing and Sanger Sequencing
● Diagnostics*
Detailed Description
Samurai Taq™ DNA Polymerase is from Thermus aquaticus. It is a thermostable enzyme with 5’-3’ DNA polymerase activity and 5'-3’ exonuclease activity, while lacking 3’-5’ exonuclease (proofreading) or endo- activity. This enzyme is a recombinant protein produced and purified from an E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Packaging: 5 units /μL
Storage Temperature: -25°C to -15°C
Taq Storage Buffer:
20 mM Tris-HCl, pH 8.0 at 25°C
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% IGEPAL CA-630
50% Glycerol
PCR Buffer (10x):
200 mM Tris-HCl, pH 8.3 at 25°C
500 mM KCl
15 mM MgCl2
| 50 Reaction Volume | Final Concentration | |
| Water (ddH2O) | adjust for 50 µl final volume | |
| 10X PCR buffer | 5 µl | 1X |
| 10 mM dNTPs | 1 µl | 0.2 mM of each |
| 10 µM Forward primer | 1.5 µl | 0.3 µM |
| 10 µM Reverse primer | 1.5 µl | 0.3 µM |
| Template DNA | Varies | < 0.02 µg/µl |
| Taq DNA polymerase | .5 µl | 2.5 U/50 µl |
| PCR cycling set-up (25-35 cycles) | |
| Initial Denaturation | 95˚C for 2 min |
| Denaturation | 95˚C for 30 sec |
| Annealing | 50˚- 68˚C for 30 sec |
| Extension | 72˚C for 1 min/kb |
| Final Extension | 72˚C for 5 min |
| Hold | 4˚C indefinitely |
Notes: DNA templates should preferably be pure. Chemical agents, ionic detergents (such as SDS) and other DNA damaging agents should be avoided. For routine applications, 25 to 35 PCR cycles is usually enough to provide sufficient copies of the target sequence.
*Usage: This product is for research use only in molecular biology applications and is not yet intended for use in diagnostic procedures. Purchase of this product does not include a license to perform any patented application; therefore it is the users responsibility to determine if any license agreement might be needed prior usage of this product. Practicing real-time require additional licensing from Roche or Applied Biosystems.
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