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Pharmozyme’s EF1 SARS-CoV-2 Detection Kit:
Our Extraction Free RT-PCR Protocol (RUO)
Redefining fast and accurate, our EF1 SARS-CoV-2 Detection Kit reduces the fastest RT-PCR protocol times in half. With automation, your lab can have a sample to answer time of one hour!
Our kit is designed for cDNA synthesis from RNA and qPCR to occur through a one-step, probe-based RT-qPCR. The thermostable (55°C) and robust reverse transcriptase enzyme have shown high efficiency and sensitivity including in samples with low RNA copy number or those with inhibitory material. Our enzyme allows cDNA synthesis from RNA with complex secondary structure.
Pharmozyme’s EF1 SARS-CoV-2 Detection kit is CE marked for in vitro diagnostics and is designed to be used with viral RNA, extracted from respiratory samples such as nasopharyngeal/ oropharyngeal aspirates or swabs.
Your Covid-19 research just got twice as fast!
Features of Pharmozyme’s EF1 SARS-CoV-2 Detection kit:
- Multiplexed reaction with E & N2 (highly conserved) target genes, producing a strong signal
- 5 min Prep – Sample directly to RT-PCR (No RNA Extraction Step)
- 1 to 1.5 hr Sample to Answer Protocol
- Includes human RNase P gene as internal control
- High throughput optimized
- The enzyme master mix will not freeze at -20°C, thus it will not require thawing and will only require minimal preparation or mixing
The kit consists of all the necessary reagents needed for the detection of SARS-CoV-2 RNA.
This includes the following:
- Master mix containing dNTPs and all the necessary additives
- Sample preparation buffer A
- Primer and probe mixture
- Positive control
- Nuclease-free water
Pharmozyme’s SARS-CoV-2 One-Step RT-qPCR kit (RUO)
Pharmozyme has assembled one the most sensitive SARS-CoV-2 One-Step RT-qPCR enzyme kits for your research.
Accurate, easy to use, and providing clear results, our kit is designed for cDNA synthesis from RNA and qPCR to occur through a one-step, probe-based RT-qPCR. The thermostable (55°C) and robust reverse transcriptase enzyme have shown high efficiency and sensitivity including in samples with low RNA copy number or those with inhibitory material. Our enzyme allows cDNA synthesis from RNA with complex secondary structure.
Pharmozyme’s SARS-CoV-2 One-Step RT-qPCR Detection kit is designed to be used with viral RNA, extracted from various respiratory samples such as nasopharyngeal/ oropharyngeal aspirates or swabs.
A kit when you can’t leave anything in your research up to chance!
Features of Pharmozyme’s SARS-CoV-2 One-Step RT-qPCR Detection kit:
- Short 70-minute per run
- Multiplexed reaction with E & N2 (highly conserved) target genes, producing a strong signal
- Includes human RNase P gene as internal control
- High throughput optimized
- The enzyme master mix will not freeze at -20°C, thus it will not require thawing and will only require minimal preparation or mixing
The kit consists of all the necessary reagents needed for the detection of SARS-CoV-2 RNA.
This includes the following:
- 2x master mix containing all enzymes*
- Buffers
- dNTPs
- Additive
- Primer and probe mixture
- Positive control
- Nuclease-free water.
*The master mix does not freeze at its storage temperature of -20°C, making reaction preparation quick and easy.
Changing COVID-19 detection research
SARS- CoV-2 One-Step RT-qPCR Detection Kit (CE-IVD for Export Only)
Specification:
- Uses highly specific primers targeting the highly conserved E and N2 regions as is recommended by the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC) in the United States
- The RT-PCR reaction can be completed in 1h and 11 min
- Limit of detection (LoD): 13 copies per reaction at Ct <38
- Clinical performance for this kit was confirmed at 100% at LoD of 13 copies/reaction at Ct <38
- Compatible with standard RT-qPCR machines (Applied Biosystems 7500, QuantStudio System, etc..)
Storage condition upon arrival: Keep in the freezer -15˚C to -25˚C
Shipping Condition: Dry Ice
